Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing

simultaneous two - photon calcium imaging at different depths with spatiotemporal multiplexing

nature methods | ADVANCE ONLINE PUBLICATION | Optical probing of neuronal activity with fluorescent calcium indicators is a powerful approach to study information processing in the brain1. In particular, calcium imaging with two-photon laser scanning microscopy (2PLSM)2 is an ideal tool for recording network activity in vivo because it is less invasive than electrophysiology, more cells can be ...

Spatiotemporal dynamics of rhythmic spinal interneurons measured with two-photon calcium imaging and coherence analysis.

In rhythmic neural circuits, a neuron often fires action potentials with a constant phase to the rhythm, a timing relationship that can be functionally significant. To characterize these phase preferences in a large-scale, cell type-specific manner, we adapted multitaper coherence analysis for two-photon calcium imaging. Analysis of simulated data showed that coherence is a simple and robust me...

Simultaneous Two-photon Optogenetics and Imaging

10 Abstract 11 The simultaneously imaging and manipulating of neural activity in three-dimensions could 12 enable the functional dissection of neural circuits. Here we have combined two-photon 13 optogenetics with simultaneous volumetric two-photon calcium imaging to manipulate neural 14 activity in mouse neocortex in vivo in 3D, while maintaining cellular resolution. Using a hybrid 15 holograp...

Denoising Two-Photon Calcium Imaging Data

Two-photon calcium imaging is now an important tool for in vivo imaging of biological systems. By enabling neuronal population imaging with subcellular resolution, this modality offers an approach for gaining a fundamental understanding of brain anatomy and physiology. Proper analysis of calcium imaging data requires denoising, that is separating the signal from complex physiological noise. To ...

Evaluation of Reflection Optical Imaging Characteristics Using Fluorescence with Near Infrared Wavelength at Different Depths of Tissue Equivalent Phantom